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Ultrastuctural features of HEp-2 cells treated or not with PI-PLC and then exposed to VacA. Cells untreated (A) or treated with PI-PLC (B) were incubated for 10 min with VacA+ BCF and then for 3 h with HBSS containing 5 mM NH4Cl (see Methods). Note large vacuoles filled with intense VacA immunoreactivity in A, to be compared with the small vacuoles and scarce VacA immunoreactivity in B. Aldehyde-osmium fixation, VacA immunogold, uranyl-lead counterstaining. Bars = 0.85 μm.
