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. 2000 Nov;11(11):3911–3923. doi: 10.1091/mbc.11.11.3911

Figure 7.

Figure 7

Type IV collagen localization in wild-type and nid-1 mutant animals. Wild-type N2 (A–D, K, L, N, P, and Q), cg118 mutant (E–H, M, O, R, and S) or cg119 mutant (I, J, T, and U) animals were stained with anti–LET-2 type IV collagen antisera (A, C, E, G, I, K, M–P, R, T) or anti-myosin A antibodies (B, D, F, H, J, L, Q, S, and U). (A and B) Wild-type twofold stage embryo showing type IV collagen localization under body wall muscles (arrow) and on the surfaces of the pharynx (p) and intestine (i). (C, D). Wild-type threefold stage embryo showing type IV collagen localization under body wall muscle quadrants (arrowheads), and on the surface of pharynx (p) and intestine (i). (E, F). Twofold stage cg118 mutant embryo showing type IV collagen localization to body wall muscles (arrow), and on the surfaces of the pharynx (p) and intestine (i). (G and H) Threefold stage cg118 mutant embryo showing type IV collagen localization under (arrowheads) and at the edges (arrow) of body wall muscle quadrants and on the surfaces of the pharynx (p) and intestine (i). (I and J) Threefold stage cg119 embryo showing type IV collagen localization under (arrowheads) and at the edges (arrow) of body wall muscle quadrants and on the surfaces of the pharynx (p) and intestine (i). (K and L) Wild-type L1 larva showing type IV collagen localization under body wall muscle (arrow), at the nerve ring (nr), and on the surfaces of the pharynx (p), intestine (i), and gonad (g). (M) L1 stage cg118 mutant larva shows type IV collagen localization under body wall muscle (arrow), at the nerve ring (nr), and on the surfaces of the pharynx (p), intestine (i), and gonad (g). (N) Wild-type L4 stage larva showing type IV collagen localization to the surface of the gonad (arrow), the distal tip cell (dt), spermatheca (st), and uterus (u). The location of the vulva (vu) is indicated. (O) L4 stage cg119 mutant larva shows type IV collagen localization to the surface of the gonad (arrow), the distal tip cell (dt), spermatheca (st), and uterus (u). vu, vulva. (P and Q) Wild-type L4 stage larva showing type IV collagen accumulation at the interfaces between muscle cells (arrows) and in a dense body pattern under the cells. (R and S) L4 stage cg118 mutant animal showing type IV collagen accumulation at the interfaces between muscle cells (arrows) and in a dense body pattern under the cells. (T and U) L4 stage cg119 mutant animal showing type IV collagen accumulation at the interfaces between muscle cells (arrows) and in a dense body pattern under the cells.