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Indirect immunofluorescence labeling of FKBP65 is identical to that of Grp94. FBCs were fixed with 2% paraformaldehyde, permeabilized with 0.1% saponin in PBS containing 1% BSA, and immunolabeled for either FKBP65 or Grp94. Both antibodies show the same ER pattern of staining, and in many cells a negatively stained area representing the perinuclear Golgi cap can be seen.
