Figure 5.

Two-hybrid interaction of Pex13-SH3 and Pex14p is reduced in the absence of wild-type Pex5p. A PCY2 strain harboring a deletion of the PEX5 gene (PCY2 pex5Δ) was transformed with expression constructs encoding either wild-type Pex5p or mutant Pex5p, or with an empty plasmid (−). The two-hybrid interaction of Pex13-SH3 and Pex14p was determined in these different strains by measuring the β-galactosidase activity. Indicated is the mean of two measurements in triplicate cultures ± SD. Background (▪) represents β-galactosidase activity in strains transformed with either GAL4DB-Pex13-SH3 or GAL4AD-Pex14p alone.