Table 2.
N-terminal pex5 mutants are selectively disturbed in two-hybrid interaction with Pex13-SH3 in HF7c
| GAL4 AD fusion
|
GAL4 DB fusion
|
|||
|---|---|---|---|---|
| Pex5 | Pex13-SH3 | Pex14p | PTS1 | Pex8p |
| Wild-type | ++ | ++ | ++ | ++ |
| N3 | +/− | ++ | ++ | ++ |
| N8 | +/- | ++ | ++ | ND |
| N19 | - | ++ | ++ | ++ |
| N84 | +/- | ++ | ++ | ND |
| N100 | +/- | ++ | ++ | ND |
Proteins were fused to GAL4 or GAL4 DB as indicated. Double transformants were grown on glucose plates lacking histidine. ++, growth after 3 d; +/-, growth after a minimum of 4 d; +/−, growth in small colonies after a minimum of 4 d; -, no growth; ND, not determined.