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. 2001 Jan;3(1):4–9. doi: 10.1038/sj.neo.7900132

Figure 4.

Figure 4

Subcellular localization of MARKL1 detected by immunocytochemistry. (A) HEK293 cells were transfected with pEGFP-MARKL1. Green fluorescent signals indicating EGFP-MARKL1 fusion protein were observed in the cytoplasm. (B) DAPI was used to indicate the nuclei of the cells shown in (A). (C, D) HEK293 cells tranfected with pFlag-MARKL1 were stained with anti-Flag monoclonal antibody and subsequently visualized by rhodamine-conjugated anti-mouse antibody. The Flag-tagged MARKL 1 protein was also detected in the cytoplasm.