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. 2001 Jan;3(1):27–32. doi: 10.1038/sj.neo.7900135

Figure 1.

Figure 1

JNK1 overexpression induces MMP-9 expression in SCC25 cells. The SCC25 line was stably transfected with human JNK1 expression vector as described in Materials and Methods section. Whole cell lysates from neomycin-resistant (neo) and JNK1-overexpressing clones (JNK1-1, -2) were subjected to Western blot using anti-JNK1 and anti-MMP-9 antibodies. Blots were stripped and probed with anti-β-actin antibody to verify equal amounts of protein in each lane. Concentrated conditioned media were also subjected to Western blotting to determine relative levels of secreted MMP-9. This experiment was performed three times with similar results. Representative blots are shown.