Figure 6.

The growth-related function of fission yeast Int6 can be supplied by human Int-6. (A) Complementation of the int6Δ growth defect by Int6 or Int6CT. The int6Δ strain was transformed with pREP3X (v) or pREP3X cDNA library plasmids encoding full-length Int6 or Int6CT as indicated. Transformants were streaked onto an EMM2 agar plate supplemented with adenine and uracil, and were photographed after four days incubation at 30°C. (B and C) The int6Δ strain was transformed with pREP3X (v) or pREP3X derivatives containing full-length human Int-6 (fl), or either of the two C-terminally truncated Int-6 derivatives indicated in Figure 1 (m, s). Transformants were streaked onto an EMM2 agar plate supplemented with adenine and uracil, and were photographed after 4 days incubation at 30°C (B). Whole cell protein extracts prepared from liquid cultures of the same transformants were subjected to Western blotting using an anti-HA antibody (C). The molecular masses (in kDa) of the three tagged Int-6 proteins are indicated.