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SDS-15% PAGE of p62 fusion proteins stained with Coomassie blue (SDS-PAGE) or transferred to nitrocellulose membrane. Blots are incubated with MAb 414 (MAB414), the serum of a healthy donor (control), sera of two patients with PBC (patients 1 and 2), and the serum of a patient with MCTD (patient 3).
