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Negative effect of CΔ50-Int6. Wild-type cells were transformed with either empty pREP41 vector (VC) or pREP41 containing either full-length int6+ gene (FL) or CΔ50 mutant int6+ (CΔ50) gene. Transformants were grown in PM medium for 12 h and were inoculated in PM medium containing 5 mM caffeine. Inset: Occasionally, some cells harboring pREP41-CΔ50 INT6 grew unusually long in caffeine containing medium.
