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. 2003 Mar;10(2):272–277. doi: 10.1128/CDLI.10.2.272-277.2003

FIG. 3.

FIG. 3.

(A) SDS-polyacrylamide gel (12% polyacrylamide) under reducing conditions of the chromatographic fractions containing the leukotoxic components after staining with silver nitrate. Lane 1, culture supernatant (BHI broth) of strain 122.25; lane 2, LukM; lane 3, LukF′-PV. The two bands around 55 and 65 kDa are due to impurities in the sample buffer brought along with β-mercaptoethanol. (B) Immunoblot of culture supernatant of strain Ch122 and affinity-purified Ab to LukM (lane 1) or affinity-purified Ab to LukF′-PV (lane 2). Lane 3, molecular mass markers in kilodaltons.