Table 3.

In Situ Hybridization and Immunohistochemical Analyses of Control and IFN-α-2a-Treated KM12L4 IFN^R-Induced Liver Metastases.

IFN-α Treatment*	In Situ Hybridization†				Immunohistochemical Assay				Tumor Cells‡		Endothelial Cells§
	bFGF	MMP-9	IL-8	VEGF	bFGF	MMP-9	IL-8	VEGF	PCNA+ (%)	TUNEL+ (%)	CD-31/TUNEL (%)
Saline	113±7	111±12	27±2	64±2	100±6	100±8	100±20	100±6	74±3	3.4±1	1.6±0.6
1000 U	89±13	109±7	31±3	74±6	83±12	72±6	111±30	90±3	47±4	4.1±08	4.9±1.8
10,000 U	36±5¶	52±6#	26±1	75±3	53±4#	47±4#	109±16	83±7	35±5#	9.9±1.5#	23.2±7.7¶

^*KM12L4 IFN^R cells (1x10⁶) were injected into the spleen of nude mice. Daily s.c. injections of saline or IFN-α-2a began on day 7. Mice were euthanized on day 42.

^†ISH and immunohistochemical analysis were performed on three livers from each treatment group. Image analysis was carried out on five areas of each section. For image analysis of ISH, expression of each factor was normalized to expression of poly d(T) in the same area. For image analysis of immunohistochemical characteristics, expression of each factor was compared to that of control sections assigned the value of 100.

^‡Percentage of dividing cells (PNCA+) and apoptotic cells (TUNEL+) was calculated by examining at least 100 nuclei/sample.

^§CD-31 (Texas Red) and TUNEL (FITC green).

^¶P<.001 versus control.

^#P<.01 versus control.