Figure 1.
In vitro binding of mot-1 and mot-2 to p53. In vitro translated 35S-labeled murine p53 (53-kDa band) and a small-sized (43-kDa) product (lane 0) were mixed with His-tagged mot-2 (lane 2) or mot-1 (lane 3) recombinant proteins and were immunoprecipitated with anti-mortalin antibodies. Immunoprecipitated mot-1 and mot-2 were detected by Western blotting with anti-His tag antibody and coimmunoprecipitated p53 was detected by autoradiography. p53, but not its carboxy-terminal truncated form coprecipitated with both mot-1 and mot-2 (lanes 2–3).