Figure 10.

Binding of AtCAP1 to Actin.

BY-2 cells (cultured for 7 days) overexpressing AtCAP1 under glucocorticoid-inducible promoter were transferred to Dex-free (−) or Dex-containing (+) medium (1 μM Dex) and cultured for 24 hr at 27°C. Total protein extracts (150 μg) were immunoprecipitated with either anti-AtCAP1 antibody (αCAP) or preimmune serum (Pre) and subjected to protein gel blot analysis using an anti-actin antibody. Twenty micrograms of total protein (crude extract) was loaded for each sample.