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Fractionation of Myc-Dpl1p. Wild-type (NY2261) and sncΔ (JG8) cells transformed with a Myc-Dpl1p overproducing plasmid were grown in selective media and then lysed in detergent-free buffer. The lysate was fractionated into P1, P10, and P100 pellet fractions and a S100 supernatant fraction. Myc-Dpl1p was detected on a Western blot prepared from equal aliquots of each fraction with an anti-Myc monoclonal antibody.
