Figure 4.

Vacuole fragmentation in snc1-M43A yeast. Wild-type (SP1), SNC1 snc2Δ (NY2264), and snc1-M43A snc2Δ (NY2265) yeast were grown to early log phase at 25°C. The cells were incubated at 37°C for 15 min with 50 mM FM4-64, washed, and chased at 37°C for an additional 45 min. FM4-64 fluorescence was visualized by using a digital imaging microscope.