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Sncp expression. A Western blot prepared from samples of equal protein concentration from wild-type (SP1), SNC1 snc2Δ (NY2264), snc1-M43A snc2Δ (NY2265), snc1 Δ SNC2 (NY2204), and snc1Δ snc2-M42A (NY2205) yeast was probed for Sncp and Ssop. The anti-Sncp antiserum may bind preferentially to Snc1p because the immunogen included an N-terminal domain of Snc1p that is poorly conserved in Snc2p.
