Figure 7.

Phenotypes arising after repression of Snc2p and Snc2-M42Ap synthesis. (A) Growth curves. Snc2p (NY2270) and Snc2-M42Ap (NY2271) were expressed in a snc1Δ snc2Δ host strain under control of the GAL1 promotor. Cells were grown to early log phase in galactose media and then shifted to glucose media at t = 0. Cell density was measured by reading the adsorbance at 600 nm and adjusted for dilution of the cultures to prepare a growth curve. (B) Snc2p expression. Snc2p expression level was measured by densitometry from a Western blot. The combined expression level of Snc1p and Snc2p in wild-type cells (SP1) was defined as 1. (C) ³⁵S-secretion. After 8 h of growth in glucose media cells were pulse labeled with [³⁵S]methionine for 20 min. Secreted proteins collected from the media by TCA precipitation and total cellular proteins prepared by glass bead lysis were resolved on a polyacrylamide gel, and detected by autoradiography.