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Assembly and disassembly of SNARE complexes containing Snc2-M42Ap. Cells expressing Snc2p (NY2204) or Snc2-M42Ap (NY2205), but not Snc1p, were lysed in the absence or presence of ATP. A Western blot of anti-Ssop immunoprecipitates was probed for coprecipitating Snc proteins (top). On a second immunoblot, an aliquot of each lysate was probed for Sncp and Ssop (bottom).
