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. 2000 Dec;11(12):4051–4065. doi: 10.1091/mbc.11.12.4051

Figure 9.

Figure 9

Suppression of the snc1-M43A growth defect by DPL1 overproduction. Various plasmids were transformed into a snc1-M43A strain (NY2258). Transformants were streaked to single colonies on a YPD plate and incubated for 3 d at 30°C. The plasmids tested included an empty high-copy number 2 μ vector (pRS425), pRS425 derivatives containing the wild-type DPL1 (pNB1048) and dpl1-K370N (pNB1049) and dpl1-K370R (pNB1050) mutant genes, a CEN plasmid containing the DPL1 gene that is present as a single copy in each cell (pNB1047), and the original plasmid containing the SSD1, DPL1, and YDR925C genes that was selected from a 2 μ genomic library (pNB1040).