Table 2.
Plasmids used in this study
| Plasmid | Description |
|---|---|
| pNB529 | GAL1 promotor and ADH1 terminator in pRS305 |
| pNB841 | SNC2-HA in pNB529 |
| pNB1028 | SNC2-HA-6K in pNB529 (from pNB841) |
| pNB1029 | snc2-M42A-HA-6K in pNB529 (from pNB841) |
| pNB1080 | SNC2 in pNB529 |
| pNB1077 | snc2-M42A in pNB529 |
| pNB1075 | SNC1-HA-6K in pNB529 |
| pNB1076 | snc1-M43A-HA-6K in pNB529 |
| Ycp50-HO | from Ira Herskowitz |
| pNB1030 | SNC2 in pRS305 |
| pNB1031 | snc2-M42A in pRS305 |
| pNB1040 | DPL1, SSD1, and YDR925C in YEplac181 (10.7 kb genomic DNA insert) |
| pNB1041 | DPL1 and YDR925C in YEplac181 (Xba1 dropout from pNB1040) |
| pNB1042 | DPL1 and SSD1 in YEplac181 (SacI-NaeI dropout from pNB1040) |
| pNB1037 | DPL1 in pBluescript KS- |
| pNB1048 | DPL1 in pRS425 |
| pNB1049 | dpl1-K370R in pRS425 |
| pNB1050 | dpl1-K370N in pRS425 |
| pNB1047 | DPL1 in pRS315 |
| pNB1038 | dpl1∷LEU2 in pBluescript KS- (from pNB1037) |
| pNB1039 | Myc-DPL1 in pRS425 (from pNB1048) |