Table 3.

DPL1 overproduction restores secretion from sncΔ cells

	p190 secretion (% wild-type)	Total protein synthesis (% wild-type)	Normalized secretiona (% wild-type)
Wild-type	100	100	100
sncΔ	23	41	58
sncΔ 2μ DPL1	81	107	67

Wild-type (SP1), sncΔ (JG8, snc1∷URA3 snc2∷ADE8 GAL1p-HA-SNC1) (Protopopov et al., 1993), and sncΔ cells overproducing DPL1 (pNB1047) were grown to early log phase at 30 °C in SC galactose media lacking tryptophan to maintain the GAL1p-HA-SNC1 plasmid or leucine to maintain the DPL1 plasmid, as appropriate. The cells were then shifted to methionine-free SC glucose media for 11 h to repress HA-Snc1p synthesis in the sncΔ cells. At this time point, the sncΔ cells have a reduced growth rate, but the sncΔ cells suppressed by DPL1 grow at the same rate as wild-type cells. Secretion of radiolabeled p190 and the rate of total protein synthesis were measured as described in Figure 5. At this time point, eight major radiolabeled proteins ranging from 30 to 190 kDa were secreted in similar relative amounts by all three strains. 

^a Secretion/synthesis × 100%.