Table 3.
Arabidopsis Proteins Copurified with the Thylakoid Membranes and Identified from the 2-D Electrophoresis Gels Shown in Figure 1
| Spot No. | Apparent Molecular Mass (kD) |
pI | Identitya | Accession No.b | MALDI-TOF Percent Coverage at 50 ppmc |
MS/MS Sequenced |
Localization and Cleavage Site Predictione
|
||
|---|---|---|---|---|---|---|---|---|---|
| TargetP | Predotar | SignalP | |||||||
| 75 | 18.7 | 5.5 | ClpS1 | 5123926 (At4g25370) | AIAWAIDEK (1) | C1/63 | P 1 | −73 PIA-QP | |
| 92 | 33.3 | 6.2 | Enoyl-acyl carrier protein reductase |
4006834 (At2g05990) | 35 | C2/74 | M 0.8 | None | |
| 111 | 19.5 | 6.5 | Fe-superoxide dismutase |
1351082 (At4g25100) | 13 | TFMTNLVSWEAVSAR (2) | No chloroplast | Neither | None |
| 72 | 18.3 | 6.1 | ROC4 isomerase | 461899 (At5g13120) | 21 | C2/67 | P 0.8 | −55-HYA-SP +47-GIA-LS |
|
| 81 | 28.5 | 4.7 | Fibrillin homolog | 7484966 (At4g22240) | 42 | C2/59 | P 1 | None | |
| 82 | 28.9 | 4.7 | Probable fibrillin | 7488105 (At4g04020) | 42 | C2/55 | P 1 | None | |
| 83 | 28.2 | 4.9 | Fibrillin CDSP34 homolog 1 + |
6729544 (At3g58010) | FFMISYLDDEILIVR (6) | C1/53 | P 1 | None | |
| Fibrillin CDSP34 homolog 2 |
2673904 (At2g42130) | LKEEYVEGMLETPTVIEEAVPEQLK (4) | C1/48 | P 1 | ±24 ASP-SR | ||||
| 84 | 26.1 | 5.4 | Similar to fibrillin | 11994325 (At3g23400) | LIPVTLGQVFQR (3) | C1/72 | P 0.8 | −18 ALL-SD +104 LVA-SV |
|
| 96 | 40.9 | 4.1 | Putative fibrillin | 3608139 (At2g35490) | 30 | C1/53 | M 0.9 | −45 YRP-KP +50 RFS-KI |
|
| 88 | 29.9 | 5.1 | CP29 A′ RNA-BP+ | 681904 (At3g53460) | 21 | C1/65 | Neither | −12 AFN-PK | |
| Putative RNA-BP | 3608147 (At2g35410) | 13 | C1/74 | P 1 | −45 SNL-SP +75 TSA-DE |
||||
| 119 | 32.1 | 4.1 | CP31 RNA-BP | 681908 (At4g24770) | 40 | C1/93 | P 1 | −85 DWA-EE +16 AMA-DS |
|
| 85 | 24.3 | 4.8 | CP29 B′ RNA-BP | 4056477 (At2g37220) | 35 | C1/47 | P 1 | −37 LSF-KL +107 AQL-AQ |
|
| 113 | 20.6 | 6.2 | Similar to RNA-BP | 4678944 (At3g52150) | 21 | C1/56 | P 0.6 | −42 SLA-GT | |
| 86 | 26.5 | 6.6 | Ribosomal S5 P | 4836870 (At1g78630) | 20 | C1/49 | P 1 | −17 LHT-RT +50 VKA-SS |
|
| 107 | 17.1 | 5.3 | Ribosomal S7 P | 7525079 | 34 | Chloroplast encoded | |||
| 87 | 26.8 | 6.8 | Ribosomal L4 P | 3914666 (At1g07320) | 41 | C2/32 | P 1 | −20 LFL-SS +25 SHQ-IP |
|
| 67 | 16.3 | 4.8 | Ribosomal L12 P | 548655 (At3g27850) | ILVDYLQDK (4) | C1/54 | P 0.8 | −21 TCA-ST +58 VEA-PE |
|
| 78 | 19.5 | 4.9 | Ribosomal L13 P | 1707008 (At2g33800) | 23 | C1/56 | P 1 | −19 VKS-SG +47 IYA-NS |
|
| 65 | 15.9 | 4.7 | Ribosomal L27 P | 9759141 (At5g40950) | 29 | C4/19 | P 1 | −27 SFL-NR | |
| 64 | 15.2 | 4.9 | Putative ribosomal recycling factor |
7523401 (At3g63190) | 31 | C2/18 | P 0.9 | +48 LIA-CS | |
| 209 | 18.9 | 9.3 | Psa-D | 4587564 (At1g03130) | 40 | C1/43 | P 1 | +43 AIR-AE | |
| 202 | 14.2 | 9.3 | Psa-E | 7443149 (At4g28750) | 47 | C2/44 | P 1 | ±22 AGA-SS | |
| 38 | 33.9 | 5.6 | FNR | 5730139 (At5g66190) | 35 | C2/64 | P 0.7 | ±48 VKA-QV | |
| 102 | 15.2 | 6.4 | RbcS (20.2) | 4204274 (At1g67090) | 21 | C3/54 | P 1 | ±18 AQA-TM | |
| 129 | 50.6 | 6.6 | RbcL (47.3) | 1944432 | 13 | Chloroplast encoded | |||
| 48 to 52 | 53.4 | 5.4 to 5.6 | CF1α (55.1) | 5881679 | 41 | Chloroplast encoded | |||
| 43 to 47 | 51.3 | 5.7 to 6.0 | CF1β (53.1) | 5881701 | 57 | Chloroplast encoded | |||
| 34 | 34.5 | 6.1 | CF1γ (53.1) | 461550 (At4g04640) | 22 | C1/42 | P 0.9 | −18 SLS-AD +43 SRA-SS |
|
| 8 | 17.8 | 5.8 | CF1δ (53.1) | 5916447 (At4g09650) | 55 | C4/48 | P 0.8 | −58 AMA-LA | |
| 102 to 53 | 15.2 | 6.3 to 6.4 | CF1ε (53.1) | 7525039 | 53 | Chloroplast encoded | |||
These proteins are located at the stromal side of the thylakoids and were released by Yeda press. Proteins were identified by MALDI-TOF MS and/or nano-ESI/MS/MS.
Identity given in NCBI completed by domain prediction found by Pfam, Blocks, Prints, Prodom, or Phi-Blast.
Accession numbers in NCBI and in MIPS (in parentheses).
Percentage of coverage at 50 ppm for the MALDI-TOF peptides.
Sequence tag obtained identified by ESI/MS/MS; other sequences were obtained (number of sequences identified in parentheses) but are not shown.
Localization, reliability class, and cleavage site prediction by TargetP (C for chloroplast), localization and score by Predotar (P for plastid, M for mitochondria), cleavage site prediction of the lumenal transit peptide by two versions of SignalP developed for Gram-negative (−) or Gram-positive (+) bacteria.