Figure 10.

Loss of Atγ-COP and AtArf1p from BY-2 Golgi Stacks.

(A) to (H) Cortical cytoplasm of two GmMan1-GFP cells at 5 min after BFA treatment.

(A) Fluorescent double labeling showing the intracellular distribution of GmMan1-GFP (green signal) and Atγ-COP labeling (red signal).

(B) to (D) High magnification views of the boxed region in (A).

(B) GmMan1-GFP signal. Several Golgi stacks show the typical BFA-induced ring-like structure.

(C) Corresponding diffuse Atγ-COP labeling signal.

(D) Merged image. Atγ-COP labeling has almost completely disappeared from the periphery of the Golgi stacks.

(E) Fluorescent double labeling showing the intracellular distribution of GmMan1-GFP (green signal) and AtArf1p labeling (red signal).

(F) to (H) High magnification views of the boxed region in (E).

(F) GmMan1-GFP signal. Several Golgi appear as ring-like structures.

(G) Corresponding AtArf1p labeling signal. The intensity of the labeling is weak, but ring-shaped halos still can be visualized.

(H) Merged image. The Atγ-COP halos are found at the periphery of most doughnut-shaped GmMan1-GFP signals.

(I) Perinuclear GmMan1-GFP pleiomorphic aggregates after 20 min of BFA treatment.

(J) Corresponding AtArf1p labeling. The signal is diffuse throughout the cytoplasm of the cells and sometimes is concentrated in the vicinity of the nucleus.

(K) By merging (I) and (J), AtArf1p labeling often was found to be distinct from the GmMan1-GFP pleiomorphic aggregates.

(L) to (P) Cortical cytoplasm of two GFP-HDEL cells 20 min after BFA treatment showing diffuse Atγ-COP (L) and AtArf1p labeling (O) together with unchanged GFP-HDEL fluorescence distribution (N). Compare untreated cells (Figure 8J). (M) and (P) show merged images.

All images were obtained by confocal microscopy from single optical sections of tobacco GmMan1-GFP or GFP-HDEL cells. Bars = 5 μm.