Figure 6.

EDS5 Expression after Pathogen Inoculation.

EDS5 and PR-1 transcript accumulation in wild-type plants at different times after inoculation with an avirulent strain of P. syringae carrying the avrRpt2 gene (A) and after inoculation with the isogenic virulent P. syringae (B). In (A), a short exposure time is shown at right to clarify overexposed parts of the film to the left. (C) shows EDS5 transcript accumulation in wild-type and eds5 mutant alleles 8 hr after inoculation with P. syringae carrying the avrRpt2 gene. The 18S rDNA probe was used to evaluate uniform loading. The experiments were repeated once with similar results. M, mock inoculation; I, pathogen inoculation.