Figure 1.

Two-hybrid interactions between full-length and truncated proteins. (A) A schematic representation of various truncated proteins tested by the two-hybrid system. A region essential for microtubule (MT) binding (amino acid residues 67–121), deduced from previously published results (Juwana et al., 1999), is marked by a line above Mal3. (B) A summary of the two-hybrid interactions between various combinations of proteins. The binding between full-length Moe1 and Mal3 produced 12 units of β-galactosidase activity. The plasmids expressing GAD-Mal3, GAD-Mal3ΔN, GAD-Mal3ΔC, LBD-Moe1, LBD-Moe1ΔN, and LBD-Moe1ΔC were pGADMAL3, pGAD-MAL3ΔN, pGAD-MAL3ΔC, pLBDMOE1, pLBD-MOE1ΔN (Chen et al., 1999), and pLBD-MOE1ΔC, respectively.