Figure 8.

Genetic and physical interaction between human Moe1 and EB1 in yeast. (A) Human Moe1 and EB1 were expressed in moe1Δ mal3Δ cells (strain ME1NML3A). These cells were spotted on YEAU plates after a series of 1:5 dilutions. The plates were incubated at either 30°C for 3 d or 20°C for 6 d. Plasmids tested were pARTCM (Empty vector control; Chang et al., 1994), pHSMOE1 (expressing human Moe1 under the control of the adh1 promoter), and pMAL3P-EB1 (expressing EB1 under the control of a mal3 promoter). (B) The two-hybrid reporter cells carrying the indicated hybrid proteins were replica-plated on medium lacking histidine. Shown here are cell patches that were able to grow in the absence of histidine, which indicates activation of the HIS3 reporter gene. The plasmids tested were pGAD-HSMOE1, pLBD-EB1, pLBD-Lamin (Vojtek et al., 1993), and pGAD-SNF4 (Fields and Song, 1989).