Figure 1.

Isolation of whole mount rims from isolated rat adipocytes. (A) Basal or insulin stimulated adipocytes were sandwiched between a poly-L-lysine-coated grid and nitrocellulose membrane. (B) Cells were mechanical disrupted by pulling the grid from the nitrocellulose membrane in a cytoskeleton stabilizing buffer in the cold and fixed immediately. (C) This resulted in patches of the thin cytoplasmic rims on the grid, which were processed further for IEM.