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SPK May Have CDPK Activity.
(A) Calcium dependence of SPK autophosphorylation. The effects of the addition of 100 μM Ca2+ (as CaCl2) or 100 μM EGTA to the reaction (+) were examined.
(B) Effects of inhibitors. Results in the presence of 1 μM (lane 1), 5 μM (lane 2), or 10 μM (lane 3) staurosporine (STA), ML-9, W-7, or with no inhibitor (CON) are shown. Each lane contained 3.0 μg of GST–SPK in the reaction mixture.
