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In Vitro Phosphorylation of GST–Suc Synthase by SPK.
In vitro phosphorylation of GST–Suc synthase (Susy) and RBE1 (RBE) in the presence or absence of GST–SPK (SPK). The presence of these proteins in the reaction (2.5 μg of GST–SPK, 3.5 μg of GST–Suc synthase, and 3.0 μg of RBE1) is indicated (+). Top, Coomassie blue–stained proteins (CBB); bottom, corresponding autoradiograms (AR).
