Figure 8.

In Vitro Phosphorylation of GST–Suc Synthase by the Crude Extract from the Immature Seed of the Wild-Type Plant and the Watery Seed of the Antisense Transformants.

Each lane contained 3.0 μg of GST–Suc synthase as the substrate. Lanes 1 and 2 contained 8.0 μg of total proteins in the crude extract from the immature seed of cv Nipponbare. Lane 2 also included 1 mM EGTA in the reaction mixture. Lane 3 contained 8.0 μg of total proteins in the crude extract from the watery seed of the antisense transformant ASPK19. Proteins resolved on a 10% SDS–polyacrylamide gel are shown in the autoradiogram.