Figure 10.

FTDP-17 tau mutant become increasingly insoluble in CHO cells. Soluble and insoluble fractions of tau from CHO cells expressing Wt and FTDP-17 mutant tau protein were isolated and evaluated by immunoblot analysis with Tau14/46 (A). Quantitative analysis (n = 3) of the percentage of insoluble tau relative to the soluble fraction in Wt-, ΔK-, and VPR-expressing CHO cells (B). There is an increase in the amount of insoluble tau present in cells expressing the ΔK and VPR tau mutants relative to Wt tau.