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RW and VPR tau mutants exhibit reduced phosphorylation at Ser396 and Ser404. The phosphorylation of Wt and mutant tau at multiple sites was determined by immunoblot analysis with a panel of anti-tau antibodies. Note that the phosphorylation of the RW and VPR tau mutants expressed in CHO cells is reduced at the Ser 396/404 site (as detected by PHF1 and T3P antibodies), but not at Thr181 (AT270), Ser262 (12E8), or Thr231 (PHF6).
