Figure 4.

Turnover of Wt and mutant tau in CHO cells is similar. Wt and mutant tau proteins were immunoprecipitated at various times after pulse labeling with [³⁵S]methionine. No significant difference in the turnover rate of mutant versus Wt tau was apparent in these cells. However, although Wt tau and PL tau mutants migrated more slowly over the chase period, an indication of increase phosphorylation, the slower migrating tau isoforms are not detected in cells harboring the RW and VPR mutations.