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. 2002 Mar;14(3):713–726. doi: 10.1105/tpc.010468

Figure 3.

Figure 3.

DNA Gel Blot Analysis of Ac Insertion Sites.

Each lane on the gel corresponds to DNA from an individual transposant digested with PstI.

(A) Diagnostic hybridization with the 858-bp Ac probe shown below. New Ac-hybridizing bands are detected in transposants 905.34, 905.29, and 905.28.

(B) Confirmation hybridization of the same membrane with the IPCR product from transposant 905.29 amplified with primers P3 and P4 (putative tac905.29 site). The probe detects a 4.6-kb larger band only in the lane containing DNA from transposant 905.29.

B, BamHI; E, EcoRI.