Table 2.

Characterization of Isolated tac Sites

No.	tac Site	Enzyme a	Sequence Homology	E Value	Expression Data	Location	Copy No.
1	tac901.04	SstI	Arabidopsis cytochrome P450	5e−40	Undetected	9: 13 cM from wxb	1
2	tac902.05	SalI	tRNA-gly (GCG)	2e−33	Constitutive	9: 29 cM from wx	1
3	tac902.07	SalI	Arabidopsis ring zinc finger	1e−17	Seedling; tassel; 3-week-old   embryo	9: 18 cM from wx	1
4	tac902.33	SacI	Rice plastid S9 ribosomal protein	6e−86	Endosperm; ear; tassel	1L, close to telo	1
5	tac903.05	SstI	No homology		Wounded or salt-treated   seedlings	Unlinked to wx	1
6	tac903.06	SstI	Arabidopsis auxin-inducible   protein	4e−6	Etiolated seedlings; light   repressed	Unlinked to wx	1
7	tac905.05	EcoRII	No homology		Undetected	9S: 7 cM from wx	1
8	tac905.29	PstI	Arabidopsis Ser Thr kinase	7e−11	Tassel; roots	9S: 9 cM from wx	1
9	tac907.25	PstI	Arabidopsis sugar transporter	1e−8	Roots	Unlinked to wx	1
10	tac907.49	PstI	No homology		Undetected	Unlinked to wx	1
11	tac907.51	NcoI	Arabidopsis short-chain ADHc	3e−33	Higher in older tissues	Unlinked to wx	1
12	tac907.54	PstI	Arabidopsis hypothetical protein	7e−25	Immature ear; 2-week-old seed	Unlinked to wx	1
13	tac909.06	EcoRI	Tobacco hypothetical protein	1e−5	Undetected	Unlinked to wx	4–5
14	tac911.14	EcoRI	Arabidopsis DNA-   3-methyladenine glycosidase	3e−27	Mature leaves; silk	Unlinked to wx	1
15	tac911.16	PstI	No homology		Undetected	Unlinked to wx	1
16	tac911.17	PstI	No homology		Undetected	Unlinked to wx	1
17	tac911.22	PstI	Maize early embryo EST	5e−10	Tassel (3 to 8 cm); stem	Unlinked to wx	1
18	tac911.28	PstI	Arabidopsis Ser Thr kinase	2e−7	Endosperm; seedlings	Unlinked to wx	3
19	tac911.30	PstI	No homology		Undetected	Unlinked to wx	1
20	tac913.01	PstI	No homology		Roots: salt induced; seed	9S: 8 cM from wx	1
21	tac913.09	PstI	Arabidopsis hypothetical protein	8e−6	Tassel (3 to 8 cm); seedling   shoot	9S: 10 cM from wx	2
22	tac913.14	PstI	No homology		Endosperm; seedling shoot;   root	9S: 12 cM from wx	1
23	tac914.07	PstI	No homology		Undetected	Unlinked to wx	1
24	tac914.11	PstI	No homology		Undetected	9S: 16 cM from wx	1
25	tac914.13	BglII	No homology		Undetected	9S: 21 cM from wx	1
26	tac914.23	PstI	Maize wx:Ac to intron 4	7e−82	Endosperm; pollen	9S: wx	1
27	tac917.09	NcoI	No homology		Immature ear	9S: 4 cM from wx	4
28	tac917.12	EcoRI	Arabidopsis NAM-like protein	3e−5	Etiolated seedlings	9S: 14 cM from wx	1
29	tac917.27	PstI	Arabidopsis auxin efflux carrier   protein	1e−5	Tassel; seedling; silk	9S: 12 cM from wx	1
30	tac917.29	PstI	No homology		Seedling shoot and root;   3-week-old embryo	9S: 8 cM from wx	1
31	tac918.02	PstI	No homology		Undetected	Unlinked to wx	1
32	tac919.27	PstI	Arabidopsis Pro-rich protein	2e−5	Tassel; stem; silk	9: 35 cM from wx	1
33	tac919.39	BglII	Barley SERK1 Leu-rich repeat   protein	1e−14	Undetected	9: 37 cM from wx	1
34	tac919.41	BglII	No homology		Etiolated seedlings	9: 32 cM from wx	1
35	tac921.20	EcoRI	Rice Arg decarboxylase	4e−5	Tassel; leaf	9S: 3 cM from wx	1
36	tac923.08	EcoRI	No homology		3-week-old embryo	Unlinked to wx	2
37	tac923.13	EcoRI	No homology		Undetected	Unlinked to wx	1
38	tac923.17	EcoRI	No homology		Immature seed; tassel	Unlinked to wx	1
39	tac929.20	EcoRI	Arabidopsis unknown protein	2e−15	Undetected	9S: 9 cM from wx	1
40	tac2094	λ	Arabidopsis MAPK kinased	e−113	Root; ear	9S: <1 cM from bz	4
41	tac6067	λ	Maize sesquiterpene cyclase	0	Leaves, volicitin induced	9S: <1 cM from bz	1
42	tac6087	λ	Maize sesquiterpene cyclase	0	Leaves, volicitin induced	9S: <1 cM from bz	1
43	tac6058	PCR	No homology		Endosperm; tassel	9S: <1 cM from bz	1
44	tac7077	EcoRII	No homology		Constitutive	9S: <1 cM from bz	2
45	tac8193	PstI	No homology		Undetected	5: 4 cM from T5-9c	1
46	tac8194	PstI	Rice zinc finger protein	2e−12	Undetected	7: 3 cM from T7-9a	1

^aRestriction enzyme used to digest genomic DNA; four tac sites were isolated by either cloning in λ or direct PCR.

^bcM, centimorgan.

^cADH, alcohol dehydrogenase.

^dMAPK, mitogen-activated protein kinase.