Table 2.
Substrate | Relative activity (%)a |
Amino group donorb | |
Putrescine | 100 |
Cadaverine | 97 |
Spermidine | 32 |
Agmatine | 2 |
L-Ornithine | 2 |
Amino group acceptorb | |
2-OG | 100 |
α-ketobutyric acid | 38 |
Pyruvic acid | 12 |
a The PATase activity with 2-OG as amino group acceptor was arbitrary defined as 100% relative activity. b 2-OG was used as amino acceptor with different polyamines and amino acid as amino group donors, and putrescine was used as amino group donor with different amino acceptors at final concentrations of 10 mM, respectively.