Figure 1.

CLV3 Protein Is Localized to the Apoplast.

(A) to (C) Phase-contrast optics used to detect GUS.

(A) Cell transiently expressing the GFP/GUS protein alone, which is confined to the nucleus (arrow).

(B) Cells transiently expressing the CLV3Δsp-GFP/GUS fusion protein, which is present throughout the cytoplasm.

(C) Cells transiently expressing the CLV3-GFP/GUS fusion protein, which is detected only between cells (arrows).

(D) Inflorescence apex of a clv3-2 mutant plant showing massive meristem enlargement (arrow) and production of supernumerary flowers and floral organs.

(E) Inflorescence apex of a clv3-2 mutant plant stably expressing the 35S::CLV3-G fusion protein. This transgenic T2 plant resembles the wild type, indicating that CLV3 function has been restored by the transgene.

(F) Seedling with a prematurely terminated meristem from a 35S::CLV3-G clv3-2 line. The shoot apical meristem has formed a terminal leaf.

(G) GFP fluorescence in a root from a 35S::CLV3-G wild-type T2 transgenic plant.

(H) GFP fluorescence in a root from a 35S::CLV3Δsp-G wild-type T2 transgenic plant.

(I) Autofluorescence in the cell wall of an untransformed wild-type hypocotyl.

(J) GFP fluorescence in the hypocotyl of a 35S::CLV3-G wild-type T2 transgenic plant.

Bars = 20 μm in (G) and (H) and 50 μm in (I) and (J).