Figure 4.

CLV3 Is Localized in the Extracellular Space.

(A) and (B) Localization of a Sec2 fusion protein was analyzed in roots of a transgenic line that showed complementation of the clv3-2 mutation.

(C) Section from an untransformed clv3-2 mutant plant showing no labeling.

(D) Section from the same plant as in (A) and (B) incubated with no primary antibody showing no labeling.

(E) Vac2 transformant with no transgene activity showing no immunolabeling in the cell wall.

(F) Vac2 transformants with complementation of the clv3-2 mutation showing immunolabeling in the cell wall.

(A) to (D) show streptavidin conjugated to 15-nm gold particles; (E) and (F) show streptavidin conjugated to 10-nm gold particles. Bars = 200 nm.