Figure 3.

Molecular Characterization of the rar1 Mutants.

(A) RNA gel blot analysis of RAR1 transcript levels. Total RNA (20 μg) from 4-week-old healthy Ler and rar1 mutant seedlings was probed with a genomic RAR1 DNA fragment. Equal loading was determined by visualization of rRNA on the filter with methylene blue.

(B) Immunoblot analysis of RAR1 protein. Total soluble protein extracts (50 μg) prepared from the same material used for the RNA analysis shown in (A) were probed with polyclonal anti-RAR1 antisera. Asterisks indicate truncated products detected in rar1-10 and rar1-12. Equal loading was determined by Ponceau S staining of the filter. Similar results were obtained in a second independent experiment.