Figure 6.
Effect of rar1 on Pathogen-Induced PR1 Transcript Accumulation.
Leaves of 5-week-old Ler, eds1-2, and rar1-10 plants were hand infiltrated with Pseudomonas DC3000 expressing avrRpm1 or DC3000 containing an empty vector in 10 mM MgCl2 or with 10 mM MgCl2 alone. Total RNA was extracted at 0, 6, 12, 24, 48, and 72 hr after infection, and 20-μg samples were probed on gel blots with a genomic PR1 DNA fragment. Equal loading was determined by visualization of rRNA on the filter with methylene blue (bottom gel). Three independent experiments gave similar results.