Figure 4.

Light-Driven Cysteinyl-Flavin Adduct Formation in LOV2.

Fourfold noncrystallographic symmetry–averaged omit maps of FMN and Cys-966 were calculated from the dark state (Crosson and Moffat, 2001) (A) and the photoexcited state (B) of phy3 LOV2. Maps are contoured at ±5σ and ±9σ, in which σ is the root-mean-square value of the electron density. The blue ribbon represents the 3₁₀ helix that contains the Cys. Atom colors are as in Figure 3. The images were generated in Ribbons (Carson, 1997).