Figure 6.

Alignment of LOV Domains with Known and Predicted Photoactivity.

Phototropin and phy3 LOV domains with known photoactivity include Arabidopsis phot1 and phot2 and maidenhair fern (Adiantum) phy3. LOV domains from proteins that regulate circadian rhythm include Arabidopsis ZTL and FKF1 and Neurospora Wc-1 and VVD. Sequences for two eubacterial proteins containing a LOV domain with a flavin binding motif and conserved Cys also are shown: Bacillus YtvA and a putative sensor kinase from Caulobacter. Flavin-interacting residues are marked above the sequences (inverted triangles), as are residues whose side chains exhibit significant motion upon photoexcitation (+). Identical (black) and similar (gray) residues are highlighted at a 50% cutoff. The secondary structure of LOV2 is marked above the alignment: β-strand (arrows) and helix (helices).