Table 3.

Reciprocal Crosses between anp1/anp1;anp2/ANP2;anp3/ANP3 and Wild Type ^a

	Gamete Frequency
	anp1; ANP2;ANP3	anp1; anp2;ANP3	anp1; ANP2;anp3	anp1; anp2;anp3
Cross					P Valueb
Female mutant
Observed	35	40	36	0	–
Expected 1	28	28	28	28	<0.0001*
Expected 2	37	37	37	0	0.95
Male mutant
Observed	94	52	71	0	–
Expected 1	54	54	54	54	<0.0001*
Expected 2	72	72	72	0	0.007*

^aGenotypes of 328 progeny from reciprocal crosses were determined by PCR and used to infer the genotype of the gamete contributed by the mutant parent in each cross. The mutant parent was anp1/anp1;anp2/ANP2;anp3/ANP3 and the other parent was fully wild type (ANP1/ANP1;ANP2/ANP2;ANP3/ANP3). The raw number of gametes observed for each genotype is shown. Expected 1 indicates the number of gametes expected when the four possible gametes are equally viable. Expected 2 indicates the number of gametes expected when the anp1;anp2;anp3 gametes are not viable. The chi-squared test was used to determine if the observed data were significantly different from each of these expected distributions.

^bSignificant P values are indicated with an asterisk.