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. 2003 Apr;77(7):4004–4014. doi: 10.1128/JVI.77.7.4004-4014.2003

FIG. 3.

FIG. 3.

Analysis of S510-518 tetramer-positive CD8+ T cells following infection with MHV. CD8+ T cells were purified from CLN of CCL3+/+ and CCL3−/− mice at days 7 and 12 p.i. by using MACS microbeads conjugated to anti-CD8 monoclonal antibody. To determine the number of CD8+ T cells specific for the immunodominant S510-518 epitope, purified CD8+ T cells were stained with tetramer. Shown are representative dot blots indicating the percentages of S510-518-positive cells compared to side scatter (y axis) present in CLN and spleen of CCL3+/+ and CCL3−/− mice. Data presented are representative of the results obtained from three separate experiments with three mice per experiment.