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. 2003 Apr;77(7):4004–4014. doi: 10.1128/JVI.77.7.4004-4014.2003

FIG. 6.

FIG. 6.

Chemokine receptor gene expression in CCL3+/+ and CCL3−/− mice. (A) CCR7, CXCR3, and CCR5 mRNA transcript levels were determined by RPA analysis of total RNA obtained from CD8+ T cells isolated from CLN of MHV-infected CCL3+/+ and CCL3−/− mice at 7 and 12 days p.i. (B and C) Densitometric analysis of chemokine receptor mRNA transcripts obtained from the scanned autoradiograph. CCL3−/− CD8+ T cells exhibited a reduction in transcript levels for CXCR3 and CCR5 and an increase in CCR7 expression at both time points. Data are the average normalized units representing the ratio of band intensity to L32 control. Nine CCL3+/+ mice and nine CCL3−/− mice were used. A similar receptor profile was observed in spleens of CCL3+/+ and CCL3−/− mice (data not shown). Sham-infected CCL3+/+ and CCL3−/− mice displayed receptor profiles similar to those of MHV-infected CCL3−/− mice at day 7 p.i. (data not shown).