TABLE 2.
Characterization of T cells used for adoptive transfera
| Mouse | Day p.i. | No. of CD8+ cells | No. of CD8+ (S510-518) tetramer-positive cells | No. of CD4+ cells | No. of CD4+ (M133-147)b cells |
|---|---|---|---|---|---|
| CCL3+/+ | 7 | 7.5 × 105 | 5.3 × 104 | 5.0 × 105 | 4.7 × 104 |
| CCL3−/− | 7 | 7.0 × 105 | 1.1 × 105 | 4.3 × 105 | 8.6 × 104 |
a
Characterization of mononuclear cells isolated from the spleens of CCL3+/+ or CCL3−/− mice following i.p. immunization with 2 × 105 PFU of MHV. A total of 5 × 106 cells were used for adoptive transfer studies. The data are representative of two separate experiments with a minimum of three mice per group.
b
The number of virus-specific CD4+ T cells was determined by their ability to produce IFN-γ in response to secondary stimulation with the CD4-immunodominant epitope M133-147. Total numbers were determined by multiplying the percentage of IFN-γ-producing cells by the total number of CD4+ T cells.