TABLE 3.
Characterization of infiltrating T cells within secondary lymphoid tissuesa
| Organ | Mouse | Day p.i. | No. of CD4+ cells | No. of CD8+ cells | No. of CD8+ (S510-518) cells |
|---|---|---|---|---|---|
| Lymph nodesb | CCL3+/+ | 7 | (8.4 ± 0.1) × 105 | (5.7 ± 0.4) × 105 | (3.3 ± 0.1) × 104 |
| 12 | (9.6 ± 0.3) × 104 | (7.2 ± 0.3) × 104 | (5.5 ± 0.8) × 103 | ||
| CCL3−/− | 7 | (8.2 ± 0.2) × 105 | (5.0 ± 0.1) × 105 | (4.0 ± 0.1) × 104 | |
| 12 | (9.1 ± 0.2) × 105 | (5.8 ± 0.2) × 105 | (1.3 ± 0.2) × 105d | ||
| Spleenc | CCL3+/+ | 7 | (7.8 ± 0.5) × 106 | (2.3 ± 0.2) × 106 | (1.6 ± 0.3) × 105 |
| 12 | (6.1 ± 0.3) × 105 | (6.6 ± 0.3) × 105 | (2.0 ± 0.4) × 104 | ||
| CCL3−/− | 7 | (5.1 ± 0.1) × 106 | (2.5 ± 0.6) × 106 | (4.2 ± 0.2) × 105d | |
| 12 | (6.3 ± 0.3) × 106 | (2.6 ± 0.3) × 106 | (7.0 ± 0.6) × 105d |
a
Total cells were determined by multiplying the percentage of doubly positive cells by the number of total lymphocytes isolated.
b
Characterization, by flow cytometric analysis, of mononuclear cells within the CLN following i.c. infection with MHV. The data are representative of three separate experiments with a minimum of three mice per group and are means ± SEM.
c
Characterization, by flow cytometric analysis, of mononuclear cells within the spleen following i.p. immunization with MHV. The data are representative of three separate experiments with a minimum of three mice per group and are means ± SEM.
d
P ≤ 0.01 compared with results for CCL3+/+ mice.