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. 2003 Apr;77(7):4401–4408. doi: 10.1128/JVI.77.7.4401-4408.2003

FIG. 3.

FIG. 3.

Inhibition of HCV RNA synthesis by the MT inhibitors colchicine and nocodazole. Effects of colchicine (colc) and nocodazole (nocod) are shown on the left and right panels of the figure, respectively. (A) Decreased HCV replicon RNA in cells treated with colchicine (1 or 0.3 μM) compared to those treated with ethanol equivalent to 1 μM colchicine (solvent) or in cells treated with nocodazole (19.9 or 13.3 μM) compared to those treated with DMSO equivalent to 19.9 μM nocodazole (solvent). (B) Morphology of the HCV replicon cells corresponding to those harvested for RNA detection for panel A. (C) Quantification of GFP in HCV replicon cells transfected with the pCMV-EMCV IRES-GFP reporter DNA in the presence of colchicine or nocodazole or the respective solvent control described for panel A. The figure design is identical to that described for Fig. 2C.