Figure 5.

Calcification in the brain of occludin −/− mice. Paraffin sections of the cerebellum (a and b) and basal ganglia (c, d, and f) were stained with hematoxylin-eosin. Numerous concentric, often laminated mineral deposits (arrowheads) were observed in the cerebellum (b) and basal ganglia (c, d, and f) in occludin −/− mice. These deposits were often localized along small vessels (arrow in d). This kind of deposit was not found in the brain of wild-type mice (a). EDX analysis of these deposits showed calcium and phosphorus peaks similar to those of hydroxyapatite (e). Bars, 100 μm for a and b, and 200 μm for c, d, and f.